Table 1.
PS-binding proteins
| Protein | Accession number |
Matched aa residues |
Freq. a |
Binding specificityb | |
|---|---|---|---|---|---|
| PS | PC | ||||
| ORF matched to protein coding sequence | |||||
| Pinin (Pnn) | NM_008891 | 187Q-306E | 1 | ~354X | ~3X |
| Vesicle transport through interaction with t-SNAREs 1B homolog (Vti1b) | NM_016800 | 39E-101T | 1 | ~37X | ~1X |
| Fibrillarin (Fbl) | NM_007991 | 1M-45G | 1 | ~24X | ~480X |
| A kinase (PRKA) anchor protein (gravin) 12 (Akap12) | NM_031185 | 345D-522G | 1 | ~1,067X | ~2X |
| RIKEN cDNA 5830404H04 gene (5830404H04Rik) | NM_174847 | 560P-654V | 3 | ~29,430X | ~7X |
| Procollagen, type XI, alpha 2 (Col11a2) | NM_009926 | 1354P-1475A | 1 | ~321X | ~6X |
| Myosin, light polypeptide kinase (Mylk) | NM_139300 | 1769K-1936G | 1 | ~88X | ~1X |
| Serum response factor binding protein 1 (Srfbp1) | NM_026040 | 110V-264E | 2c | ~577X | ~4X |
| PRP40 pre-mRNA processing factor 40 homolog A (yeast) (Prpf40a) | NM_018785 | 243E-401K | 1 | ~666X | ~1X |
| C2 calcium-dependent domain containing 2-like (C2cd2l) | NM_027909 | 467R-694S | 1 | ~725X | ~8X |
| RIKEN cDNA 2500003M10 gene (2500003M10Rik) | NM_023215 | 1M-96R | 2c | ~1,367X | ~4X |
| A kinase (PRKA) anchor protein 8-like (Akap8l) | NM_017476 | 274T-311G | 1 | ~248X | ~7X |
| RIKEN cDNA 1200014J11 gene (1200014J11Rik) | NM_025818 | 186K-345E | 1 | ~12X | ~1X |
| Control phage with no cDNA insert | ~1X | ~1X | |||
| Non-ORF | 0 | ||||
a
Clone frequency. All identified PS-binding phage clones encodes proteins with ORFs.
b
Binding specificity is the pfu ratio of clonal phage binding to PS or PC versus to the mock coated wells. Phage binding activities were representatives of three independent experiments.
c
Independent phage clones with overlapping, but not identical, amino acid sequences. Three redundant clones for 5830404H04Rik (NM_174847) were identical.