Table 2.
Technical performance of the KS capture OA-1 ARGS ELISA
| Linear measuring range of standard | 0.02 to 1 pmol/ml | |||
| Minimal dilution of SF | 1:50 | |||
| Minimal detectable concentration in neat SF | 1 pmol/ml SF | |||
| Intra assay CV (n = 10) | 6.1% | |||
| *Inter assay, intra lot CV (n = 5, 1st lot) | 12.2% | |||
| *Inter assay, intra lot CV (n = 23, 2nd lot) | 15.6% | |||
| Inter assay, inter lot CV (n = 28) | 19.7% | |||
| Dilution of SF | 1:50 | 1:400 | 1:800 | 1:1600 |
| Spiking recovery (mean; range) | 116%; 109 to 121% |
93%; 75 to 104% |
81%; 75 to 88% |
104%; 98 to 113% |
* Between assay variation in lot numbers (1st, #5L21/1; 2nd, #7F25/1) of the KS capture plates from Biosource.
CV = coefficient of variation; KS = keratan sulfate; SF = synovial fluid.