Table 5.
Fold change and threshold cycle for metalloproteases showing significant difference between OA and NOF
| Gene name | Gene symbol | P value | Fold change OA/NOF | Median threshold cycle |
| ADAM22 | ADAM22 | 0.00005 | 6.0 | 32.3 |
| ADAMTS2 | ADAMTS2 | 0.00005 | 8.1 | 29.1 |
| Aminopeptidase-like 1 | NPEPL1 | 0.00005 | 3.7 | 30.2 |
| Stromelysin 1 | MMP3 | 0.00005 | 0.03 | 22.5 |
| Stromelysin 3 | MMP11 | 0.00005 | 81.4 | 31.0 |
| MMP23A/B | MMP23A/B | 0.00006 | 24.7 | 34.2 |
| Mammalian tolloid-like 1 protein | TLL1 | 0.00006 | 4.9 | 31.9 |
| ADAMTS1 | ADAMTS1 | 0.00008 | 0.37 | 26.3 |
| Carboxypeptidase Z | CPZ | 0.00008 | 12.6 | 35.0 |
| Procollagen C-proteinase | BMP1 | 0.00011 | 2.9 | 29.2 |
| MT3-MMP | MMP16 | 0.00014 | 6.9 | 31.2 |
| ADAMTS14 | ADAMTS14 | 0.00017 | 11.2 | 33.5 |
| ADAMTS16 | ADAMTS16 | 0.00017 | 19.6 | 33.7 |
| Aminopeptidase N | ANPEP | 0.00017 | 5.3 | 27.2 |
| Dihydropyrimidinase-related protein 2 | DPYSL2 | 0.00022 | 2.2 | 25.1 |
| Pappalysin-2 | PAPPA2 | 0.00022 | 0.28 | 30.4 |
| Plasma Glu-carboxypeptidase | PGCP | 0.00022 | 2.9 | 29.9 |
| Cytosol alanyl aminopeptidase | NPEPPS | 0.00028 | 0.69 | 25.7 |
| Gelatinase A | MMP2 | 0.00036 | 9.7 | 24.4 |
| Leucyl aminopeptidase | LAP3 | 0.00045 | 1.6 | 28.9 |
| ADAMTS9 | ADAMTS9 | 0.00057 | 0.10 | 30.4 |
| Gelatinase B | MMP9 | 0.00057 | 31.9 | 31.3 |
| Aminopeptidase B-like 1 | RNPEPL1 | 0.00071 | 2.1 | 29.5 |
| Membrane dipeptidase 2 | DPEP2 | 0.00071 | 5.6 | 35.1 |
| NAALADASE II | NAALAD2 | 0.00071 | 3.0 | 35.0 |
| PM20D2 peptidase | PM20D2 | 0.00071 | 0.36 | 27.6 |
| ADAM12 | ADAM12 | 0.00089 | 4.0 | 27.6 |
| MMP19 | MMP19 | 0.00089 | 16.5 | 29.8 |
| MMP21 | MMP21 | 0.00089 | 3.2 | 34.1 |
| Carboxypeptidase X1 | CPXM1 | 0.00137 | 36.1 | 31.6 |
| ADAMTS12 | ADAMTS12 | 0.00170 | 20.3 | 32.3 |
| Adipocyte-enhanced binding protein 1 | AEBP1 | 0.00170 | 2.2 | 24.6 |
| Collagenase 3 | MMP13 | 0.00170 | 26.0 | 27.1 |
| ADAM9 | ADAM9 | 0.00256 | 1.8 | 27.3 |
| FACE-2/RCE1 | FACE2 | 0.00256 | 0.80 | 29.6 |
| X-Pro dipeptidase | PEPD | 0.00256 | 2.1 | 31.2 |
| Leukotriene A4 hydrolase | LTA4H | 0.00313 | 0.51 | 26.7 |
| NAALADASE like 2 | NAALADL2 | 0.00313 | 3.8 | 31.0 |
| Neprilysin | MME | 0.00313 | 9.8 | 30.9 |
| ADAM8 | ADAM8 | 0.00464 | 2.0 | 34.1 |
| Aminoacylase | ACY1 | 0.00464 | 1.6 | 33.2 |
| O-Sialoglycoprotein endopeptide | OSGEP | 0.00464 | 1.8 | 29.7 |
| ADAMTS7 | ADAMTS7 | 0.00561 | 15.0 | 34.0 |
| Dihydroorotase | CAD | 0.00561 | 1.4 | 28.1 |
| ADAMTS6 | ADAMTS6 | 0.00677 | 2.0 | 31.3 |
| FACE-1/ZMPSTE24 | FACE1 | 0.00677 | 1.8 | 30.9 |
| Glutaminyl cyclase 2 | QPCTL | 0.00677 | 0.65 | 29.4 |
| OMA1 | OMA1 | 0.00813 | 0.50 | 27.6 |
| Archaemetzincin-1 | AMZ1 | 0.00974 | 0.58 | 31.4 |
| Collagenase 1 | MMP1 | 0.00974 | 0.24 | 30.8 |
| Neurolysin | NLN | 0.00974 | 1.6 | 30.7 |
Fold change and median threshold cycle for all metalloprotease genes showing significant difference at P < 0.01 between osteoarthritis (OA) versus fracture to the neck of femur (NOF). P value from the Mann-Whitney U test. ADAM, a disintegrin and metalloproteinase domain; ADAMTS, a disintegrin and metalloproteinase domain with thrombospondin motifs; MMP, matrix metalloproteinase.