. 2009 Jul 29;4(7):e6414. doi: 10.1371/journal.pone.0006414

Table 1. In vitro expansion of CD3⁻, CD172a⁺ cells within PBMC cultures from M. bovis infected cattle stimulated with ESAT-6/CFP-10.

Group	Media only	rMPB83	ESAT-6/CFP10 peptides	rESAT-6:CFP10
Non-Infected (n = 3)	3.1 (1.4)	4.1 (1.7)	1.4 (0.4)	2.2 (1.0)
BCG-vaccinated (n = 3)	3.0 (1.0)	3.0 (0.6)	2.2 (0.4)	2.6 (0.7)
ΔRD1-vaccinated (n = 3)	2.6 (1.1)	4.7 (1.5)	2.1 (0.5)	1.9 (0.8)
*M. bovis* -infected (n = 3)	2.5 (0.2)	3.8 (0.6)	11.7 (1.7)**	10.5 (0.9)**

Data are presented as mean (±standard error) percent of CD3⁻, CD172a⁺, PKH67^lo cells (R2 gate in Fig. 1A). Isolated mononuclear cells were stained with PKH67 (a green fluorescent dye used for cell proliferation analysis), cultured for 6 days with or without stimulation as indicated in the upper margin, and analyzed by flow cytometry for phenotype and PKH67 staining intensity. Both BCG and ΔRD-1 attenuated M. bovis vaccine strains lack ESAT-6, CFP-10, and select ESX-1 secretion apparatus genes; thus, these strains do not produce ESAT-6 or CFP-10. In contrast to ESAT-6/CFP10, stimulation with MPB83, another immunodominant antigen of M. bovis, does not result in expansion of CD172a⁺ cells.

Table 1. In vitro expansion of CD3−, CD172a+ cells within PBMC cultures from M. bovis infected cattle stimulated with ESAT-6/CFP-10.

Table 1. In vitro expansion of CD3⁻, CD172a⁺ cells within PBMC cultures from M. bovis infected cattle stimulated with ESAT-6/CFP-10.