Figure 3. Antigen-specific CTL response.

(A) OT-1 and (B) 2C splenocytes stimulated with ^SIINK^b and ^SIYK^b dimeric constructs. For both positively stimulated splenocyte populations (OT-1 with ^SIINK^b and 2C with ^SIYK^b), extracellular acidification began at ~40 s. Different devices were used for each measurement. (C) OT-1 splenocytes were diluted at various ratios with wild-type B6 splenocytes; CTL response to stimulation with ^SIINK^b was measured. The |I_SD| values before and after the onset of extracellular acidification are significantly different at the 99.9% confidence level for all dilutions (t test). The same device was used for all measurements. No significant response was observed for a 1:1000 dilution. The inset shows a plot of the observed change in |I_SD| versus the OT-1 cell concentration for these measurements.