Figure 3. Regulation of the MICA 3′UTR by IFNγ-induced miR-520b.

(A) miRNA transcripts indicated on the x-axis were quantified by qPCR from total RNA isolated from cells treated with 100 U/ml IFNγ for 1 or 24 hours. Shown are 95% confidence intervals. All results were reproduced at least once for each cell line. (B) MelJuSo and HeLa cells were transfected with pMIR-MICA-3′UTR or pMIR-luc along with 5 nM of mir-520b, anti-520b, control scrambled miR, or control scrambled anti-miR. Values are the result of raw firefly luciferase values over raw renilla values which were normalized to control vector (pMIR-luc). Error bars represent standard deviation. All results were reproduced at least once for each cell line and once with MDA-MB231 cells.