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. 2009 Jun 9;100(12):1879–1888. doi: 10.1038/sj.bjc.6605034

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Copyright © 2009 Cancer Research UK

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GSI1 induces G2/M cell cycle arrest and triggers apoptosis in breast cancer cells. (A, B) Cell cycle analyses of breast cancer cell lines treated with GSI1 for 24 h (A) and 48 h (B). Cells were stained with propidium iodide and gated according to their fluorescence to differentiate cell cycle phases: 1, apoptotic cells; 2, cells in G0/G1; 3, cells in S phase; 4, cells in G2-M. Data shown are a representative of two independent experiments. (C, D) Western blot analyses were used to verify the modulation of the apoptotic response due to GSI1 treatment. (C) Three different breast cancer cell lines treated with a single GSI1 concentration (0.75 μM) for 48 h. (D) MCF-7 cells treated for 48 h with increasing concentrations of GSI1. Tubulin was used as a loading control. Note the slight effect of GSI1 on tubulin levels in MDA-MB-231 and ZR-75-1 cells (C).