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. 2009 Jun 2;100(12):1937–1942. doi: 10.1038/sj.bjc.6605085

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Copyright © 2009 Cancer Research UK

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The mean value of ID1 mRNA expression normalised GAPDH in bone marrow (A) and peripheral blood (B) according to staging classification. Group stage I consisted of patients with tumours that invaded less than the sub-mucosal layer and no lymph node metastasis (BM: n=76; PB: n=50). Group stage II consisted of patients with tumours that penetrated serosa or lymph node metastasis (Group 1) (BM: n=60; PB: n=49). Group stage III consisted of patients with tumours invasion of adjacent structures or lymph node metastasis (Group 2 or 3) (BM: n=62; PB: n=46). Group stage IV consisted of patients with distant metastasis (BM: n=91; PB: n=51). The mean value of ID1 mRNA expression in bone marrow and peripheral blood increased along with the progression of stage. The RT–PCR analysis of four representative bone marrow samples was performed in each stage (C: upper; ID1 product size 329 bp, lower; GAPDH product size 270 bp).