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. 2009 May 19;100(12):1957–1965. doi: 10.1038/sj.bjc.6605060

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Copyright © 2009 Cancer Research UK

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Notch pathway molecules are overexpressed in osteosarcoma patient specimens. Total RNA extracted from osteosarcoma biopsy specimens was used for real-time PCR. Ten of ten human biopsy specimens of osteosarcoma increased Notch2 1.3–57.3-fold. Notch1 was decreased 0.03–0.86-fold in 9 of 10 biopsy specimens. Jagged1 was upregulated 3.6–309-fold in 10 of 10 biopsy specimens. In 9 of 10 human biopsy specimens, DLL1 was decreased 0.02–0.35-fold. HES1 was upregulated in 6 of 10 and downregulated in 4 of 10 biopsy specimens. HEY1 was upregulated 1.6–12-fold in 8 of 10 biopsy specimens. HEY2 was upregulated 2.9–106-fold in 9 of 10 biopsy specimens. The comparative C_t (ΔΔC_t) method was used to determine fold change in expression using βII-microglobulin. Each sample was run minimally at three concentrations in triplicate.