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. Author manuscript; available in PMC: 2009 Jul 22.
Published in final edited form as: Protein Expr Purif. 2006 Mar 15;48(2):215–223. doi: 10.1016/j.pep.2006.02.015

Fig. 2.

Fig. 2

The polycistronic plasmids used for co-expression of mSNAPCγ4. The pCDF43/50-1 expression plasmid contains the full-length SNAP50 ORF in the first multiple cloning site and the full-length SNAP43 ORF in the second multiple cloning site. This plasmid carries the CDF origin of replication and provides streptomycin resistance. The pRSF19-1 expression plasmid contains the SNAP19 ORF in the first multiple cloning site. The second multiple cloning site was not used in this strategy. This plasmid carries the RSF origin of replication and provides kanamycin resistance.