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. Author manuscript; available in PMC: 2009 Jul 22.
Published in final edited form as: Cancer Res. 2008 Aug 1;68(15):6100–6108. doi: 10.1158/0008-5472.CAN-08-0540

Figure 5. Effects of NGAL on proliferation, apoptosis, and the sensitivity of PaCa cells to chemotherapy.

Figure 5

A. PaCa cells proliferation overexpressing NGAL or NGAL-shRNA (Suppl. Fig. 3A) was determined by using the CellTiter Aqueous One Solution Cell Proliferation Assay kit as described in Materials and Methods. B. PaCa cells apoptosis was determined by flow cytometry with Annexin V-FITC staining as described in Materials and Methods. C. Western blots show the expression of cell proliferation- and apoptosis-related proteins in stable PaCa cells that overexpress either NGAL or NGALshRNA. D. Cells were treated with gemcitabine at indicated doses for 72 h in MIANGAL and control cells or 48 h in BxPC-shNGAL and control cells (Suppl. Fig. 3B). The viability of cells was measured by using the CellTiter Aqueous One Solution Cell Proliferation Assay kit as described in Materials and Methods. Values are means ± SE of 3 independent experiments.