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. 2009 Jun 23;7:50. doi: 10.1186/1479-5876-7-50

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Copyright © 2009 Milani et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Phenotypic changes of the effector cells before (black bars) and after (grey bars) in vitro TKD/IL-2 stimulation. The percentage (left panel) and mean fluorescence intensity (mfi, right panel) values of CD3⁺T cells and CD3^-/CD56⁺and CD3^-/CD94⁺NK cells were determined before and after a 4 days in vitro TKD/IL-2 stimulation by flow cytometry. The hatched bars indicate T and NK cell values derived from the patients blood during the therapeutic break 2 (L6+2), 6 (L6+6), 8 (L6+8), and 12 (L6+12) weeks after re-infusion cycle L6. Only viable, propidium-iodide negative cells were gated and analyzed.