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View full-text article in PMC

. 2009 Jun 23;7:50. doi: 10.1186/1479-5876-7-50

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Copyright © 2009 Milani et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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In vitro cytolytic activity of patient-derived PBMNC derived from L1 to L6 and L7 to L9 against K562 cells (A) and autologous tumor (B). The lytic activity of patient-derived PBMNC before and after stimulation with TKD/IL-2 was determined by standard granzyme B ELISPOT. Filled circles indicate the cytolytic activity of unstimulated PBMNC, open circles that of TKD/IL-2 stimulated PBMNC. Due to technical problems data from L2 are not available. Viability of the tumor target cells in each assay was > 95%.