Figure 2.

Illustration of BrdUrd-labeled cells in the dentate gyrus. Photomicrographs of BrdUrd immunoreactivity in frontal section of the dentate gyrus in a control (a) and a prenatally stressed (b) rat . (Scale = 1.5 cm for 500 μm.) (c) Confocal illustration of BrdUrd-labeled cells in the dentate gyrus showing that newborn cells were mainly neurons. Indeed, BrdUrd-IR cells (red nuclear stain, Cy3) colocalized with the neuronal marker NeuN (green stain) as indicated by the yellow nuclear staining. (d) Newly born cells are rarely astrocytes because BrdUrd-IR cells (red nuclear stain) is not stained for the astroglial marker GFAP (green stain).