Table 2.
DNA binding kinetics and affinities of f-PlnC and f-PlnD.
| Analyte | Ligand | Ka1 (M-1s-1) |
Kd1 (s-1) |
Ka2 (M-1s-1) |
Kd2 (s-1) |
aKD1 (M) |
KD2 (M) |
KD1/KD2 |
| f-PlnC | PplnA | 6.8 ± 6.1 × 102 | 1.0 ± 0.1 × 10-3 | 1.5 × 105 | 1.0 × 10-3 | 1.5 ± 5.3 × 10-6 | 6.7 ± 0.1 × 10-9 | 2.2 × 102 |
| PplnM | 3.9 ± 0.9 × 103 | 1.5 ± 0.1 × 10-3 | 2.3 ± 0.1 × 105 | 7.5 ± 0.5 × 10-4 | 3.8 ± 0.6 × 10-7 | 3.2 ± 0.3 × 10-9 | 1.2 × 102 | |
| PplnJ | NA | NA | NA | NA | NA | NA | ||
| PplnE | 1.2 ± 0.9 × 104 | 2.5 ± 0.1 × 10-3 | 2.4 ± 0.1 × 105 | 2.6 ± 0.5 × 10-4 | 2.1 ± 0.2 × 10-7 | 1.1 ± 0.3 × 10-9 | 1.9 × 102 | |
| bPplnG | 8.7 ± 7.1 × 103 | 1.5 ± 0.4 × 10-3 | 1.3 ± 0.6 × 104 | 1.3 ± 0.1 × 10-3 | 1.7 ± 3.9 × 10-7 | 1.0 ± 0.5 × 10-7 | 1.7 | |
| PplnAΔL | 3.2 ± 1.2 × 104 | 9.6 ± 0.1 × 10-3 | 3.0 ± 1.3 × 10-7 | |||||
| f-PlnD | PplnA | 3.4 × 103 | 5.0 × 10-3 | 3.4 ± 0.1 × 103 | 5.1 ± × 10-3 | 1.5 × 10-6 | 1.5 ± 0.1 × 10-6 | 1.0 |
| PplnM | 3.9 × 103 | 2.2 × 10-3 | 1.4 × 104 | 1.1 ± 0.3 × 10-6 | 5.6 × 10-7 | 7.8 ± 0.3 × 10-11 | 7.2 × 103 | |
| PplnJ | 9.5 ± 0.3 × 102 | 1.8 ± 0.1 × 10-2 | 2.3 ± 0.1 × 102 | 2.2 ± 0.3 × 10-5 | 1.2 ± 0.4 × 10-5 | 9.5 ± 1.4 × 10-8 | 1.3 × 102 | |
| PplnE | NA | NA | NA | NA | NA | NA | ||
| PplnG | 1.1 ± 0.1 × 103 | 1.5 ± 0.1 × 10-2 | 2.5 ± 0.1 × 102 | 9.3 ± 8.7 × 10-6 | 1.4 ± 1.1 × 10-5 | 3.7 ± 2.3 × 10-8 | 3.8 × 102 | |
| PplnAΔL | NA | NA | NA | NA | NA | NA |
a KD1 = kd1/ka1, KD2 = kd2/ka2
b The ka was fitted locally
NA: not available