FIGURE 1 .

An age-related defect in LAT phosphorylation is reversed by vitamin E in CD4⁺ T cells isolated from the spleens of young and aged mice. (A) Lysates were serially Western blotted for LAT pY191, total LAT, and β-actin. One of 5 representative experiments is shown. (B) Densitometry was performed and the phosphorylated LAT signal was normalized to the total LAT signal. (C) Densitometry was performed and the total LAT signal was normalized to the β-actin signal. Ratios are the mean ± SEM, n = 5. *Different from young, P < 0.05 (Kruskal-Wallis nonparametric test); ^#different from vehicle control, P < 0.05 (Wilcoxon's Signed Rank t test).