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. 2008 Dec 9;122(1):114–125. doi: 10.1242/jcs.036673

Fig. 1.

Fig. 1.

RBO facilitates FM1-43-dye endocytosis at the neuromuscular synapse. (A) Schematic of dye-loading, temperature and imaging protocols used at the larval NMJ. In protocol 1, FM1-43 was loaded for 2 minutes with 60 mM [K+] at 25°C and then unloaded for 2 minutes with 60 mM [K+] at 37°C. In protocol 2, following a 10-minute incubation at 37°C, dye was loaded for 2 minutes at 37°C and then unloaded for 2 minutes at 25°C. (B) Representative images of wild type (OR), syx3-69 and rbots single mutants, and the rbots;syx3-69 double mutant following protocol 1. (C) Top three panels show representative images comparing FM1-43 loading and unloading following protocol 2. Bottom panel shows rbots;syx3-69 37°C unloading after 5 minutes of loading at 25°C. Scale bars: 10 μm. (D) Quantification of loading (L) and unloading (UL) with both protocols. Error bars show mean ± s.e.m. ***Significance of P<0.001.