Fig. 2.

Endocytic defects in rbo^ts mutants are fully rescued by the wild-type rbo gene. (A) Representative images of rbo^ts and rbo^ts/rbo²; rbo-egfp/rbo-egfp (rescue) larval NMJs loaded with FM1-43 dye after 10 minutes at 37°C (see Fig. 1, protocol 2). Scale bar: 10 μm. (B) Magnified loaded synaptic boutons in both genotypes. The wild-type rbo transgene rescues the rbo^ts dye-loading defect. Scale bar: 5 μm. (C) Quantification of dye loading in wild-type (OR) and rbo^ts compared with rescue animals.