Fig. 4.

LRP1 gene-silencing blocks MV uptake by oligodendrocytes. Oligodendrocytes were transiently transfected with NTC or LRP1-specific siRNA. (A) LRP1 expression at the protein level was assessed 36 hours after silencing by immunoblot analysis with LRP1-specific antibody, 11H4. Blots were also probed for tubulin as a control for load. (B) Oligodendrocytes were incubated with FITC-labeled MVs for 30 minutes. After washing and protease treatment, internalized MVs were detected by flow cytometry.