Fig. 1.

btn1 is required for vacuole protein sorting of the vacuole peptidase Cpy1p. Colony blot assay of indicated cells grown for 6 hours on YES agar using S. pombe specific anti-Cpy1p antibody. btn1Δ cells abnormally secrete Cpy1p. (A) Colony blot of indicated cells showing minimal Cpy1 secretion from wild-type (wt) cells but secretion from btn1Δ, vps26Δ and vps10Δ cells. (B) Bar chart depicting mean (± s.d.) density values relative to wild-type levels of Cpy1p secretion in colony blot assays (mean of three experiments).