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. 2009 Mar 19;122(8):1163–1173. doi: 10.1242/jcs.038323

Fig. 5.

Fig. 5.

Btn1p regulates Golgi number and positioning. Absence of btn1 causes a reduction in the number of Gms1p-stained Golgi compartments. (A) Confocal images of wild-type (wt) btn1Δ cells expressing Gms1p-CFP showing reduced numbers of Golgi and positioning defects (end panel shows a single typical cell). (B) Bar chart of numbers of Golgi in wild-type (wt), btn1Δ cells and wild-type cells overexpressing Btn1p (n>200 Golgi across single cross-sections in three independent experiments); wild-type cells, 7.1±2.2; btn1Δ cells, 4.2±1.4; cells overexpressing Btn1p, 5.9±2.59; ***P<0.001. (C) Absence of btn1 or ectopic expression of Btn1p alters the distribution of Golgi compartments. Bar chart of positions of Gms1p-stained Golgi compartments in wild-type (wt), btn1Δ cells and cells overexpressing Btn1p with respect to their distance from the nucleus. The cartoon in A illustrates the positions of regions counted (n>200 Golgi over three experiments). Scale bars: 10 μm.