Fig. 7.

btn1 might be required for other vps pathways. (A) Btn1 might also be involved in a second vacuole protein-sorting pathway for Cpy1p. Colony blot assay of indicated cells grown on YES agar plates showing increased Cpy1p secretion from a vps10Δbtn1Δ double deletion strain compared to vps10Δ. (B) Colony blot assay of indicated double deletion strains showing that vps26Δ and vps26Δbtn1Δ cells secrete similar amounts to parental strains, but that vps34Δbtn1Δ cells secrete more than parental strains as observed in C at a lower exposure. (D) Bar chart depicting mean density values relative to wild-type levels of Cpy1p secretion in colony blot assays (mean ± s.d. of three experiments). ^*P<0.05.