Fig. 2.

Bootstrapped phylogenetic tree of CGTases and homologous proteins. The scale bar corresponds to a genetic distance of 0.1 substitution per position. CGTases displaying >95% similarity were not included. The main type of cyclodextrin formed by the functional CGTases is indicated by α, β, or γ. a ATase (Leemhuis et al. 2004b), b Novamyl (Dauter et al. 1999), p putative CGTase, r CGTase from Archaea. (See Table S1 of the Electronic supplementary material for protein accession numbers.)