Table 4. Comparisons between fold-change in gene expression determined by microarray and qRT-PCR methods.
| MICROARRAY | qPCR | |||
| Gene | DM | DM+SERCA | DM | DM+SERCA |
| Akt1 | 0.87 | 1.43 | 0.29 | 0.74 |
| Akt2 | 1.02 | 2.1 | 0.19 | 0.77 |
| PI3Ka | 1.01 | 1.41 | 1.30 | 3.16 |
| GSK-3b | 1.08 | 0.68 | 1.10 | 0.76 |
| PKC-λ | 1.01 | 1.65 | 0.88 | 1.68 |
| GLUT1 | 0.94 | 0.68 | 1.27 | 0.61 |
| GLUT4 | 1.30 | 1.72 | 0.21 | 0.91 |
| CK | 0.88 | 1.41 | 0.59 | 2.14 |
| Serca2a | 0.79 | 1.08 | 0.48 | 2.21 |
| S100A3 | 1.80 | 0.68 | 2.63 | 1.15 |
| CaBP1 | 1.13 | 0.12 | 1.96 | 0.98 |
| RyR2 | 11.4 | 0.05 | 1.19 | 0.94 |
| Cacna1c | 1.05 | 1.55 | 0.60 | 1.12 |
| Cacnb3 | 0.29 | 0.62 | N/A | N/A |
| MMP7 | 1.63 | 0.13 | 7.08 | 0.65 |
| FZD4 | 2.32 | 0.43 | 1.50 | 0.99 |
| FGF4 | 0.24 | 0.62 | N/A | N/A |
| sFRP4 | 0.26 | 0.78 | 1.64 | 2.94 |
| Retnla | 54.03 | 0.27 | 23.8 | 0.95 |
The fold changes in mRNA levels in diabetes (DM) and DM+SERCA2a samples of selected genes were determined by microarray and RT-qPCR. Transcripts were selected based on their roles in insulin signaling, energy/metabolism, Ca2+ handling, structural remodeling and intracellular signaling. CK, creatine kinase; sFRP4, secreted frizzled-related protein 4; Retnla, resistin-like alpha; Cacna1c, calcium channel, voltage-dependent, L type, alpha 1C subunit; Cacnb3, calcium channel, voltage-dependent, beta 3 subunit. N/A, not amplified.