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. 2009 Jul 30;4(7):e6442. doi: 10.1371/journal.pone.0006442

Figure 5. A mir168a loss-of-function mutant has reduced miR168 accumulation and increased AGO1 levels.

Figure 5

(A) miR168 accumulation in wildtype Ler and the mir168a-2 mutant. RNA gel blot analysis of 10 µg of RNA with a probe complementary to miR168. U6 serves as a loading control. (B) AGO1 mRNA accumulation in wildtype Ler and the mir168a-2 mutant. RNA gel blot analysis of 10 µg of RNA with a T7-transcribed RNA probe complementary to AGO1. Ethidium bromide staining of 25S rRNA is shown as a loading control. (C) miR168 accumulation in wildtype Ler, the mir168a-2 mutant and the mir168a-2 transformed with the 4m-AGO1 construct. RNA gel blot analysis of 10 µg of RNA with a probe complementary to miR168. U6 serves as a loading control.