Figure 3. Effect of punicic acid on neutrophil degranulation.

Human neutrophils from healthy donors were pretreated (open bars) or not (black bars) with punicic acid for 30 min, challenged with TNFα or cytochalasin B, then stimulated with fMLP for 3 min. Cells were centrifuged and MPO activity was determined in the supernatants using H₂O₂ and TMB as described in Materials and Methods. MPO activity was expressed in MU/min relative to a standard curve established with a known number of neutrophils. Data are representative of 4 different experiments. * P<0.05 vs untreated cells.