Figure 4. Punicic acid (PA) inhibits TNFα-induced ROS over-production by rat neutrophils.

(A) Rat neutrophils (5×10⁵ cells in 0.5 ml Hanks buffer) were incubated in the absence or the presence of PA (10 µM) for 30 min; TNFα (10 ng/ml) was added and ROS production was measured by chemiluminescence technique in the presence of 10 µM luminol. (B) Rat neutrophils (5×10⁵ cells in 0.5 ml Hanks buffer) were incubated in the absence or the presence of PA (10 µM) for 30 min; rat TNFα (10 ng/ml) was added for 20 min before stimulation with fMLP (10⁻⁷ M). ROS production was measured by chemiluminescence technique in the presence of 10 µM luminol. (C) Total chemiluminescence in each condition was quantified and presented as mean+/−SEM of 3 experiments (PA 10 µM). * P<0.05 treated vs untreated cells.