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. 2009 Jul 6;2:5. doi: 10.1186/1755-8417-2-5

Figure 2.

Figure 2

Immunolocalization in palate tissue. a. Coronal sections through the palate of E15.5 wild-type (WT) and homozygote (Jf/Jf) embryos, immunohistochemically stained with antibodies against Fbox11, TGF-β3, TGFβR-I, Smad2, and Smad4. Scale bar 50 μm. Medial edge epithelium (mee), nasal palatal epithelium (ne) and oral palatal epithelium (oe). Graph: comparison of the percentage of epithelial cells positive for Smad2 in E15.5 wild-type and homozygote palates. b. Coronal sections through the palate of E14.5 (before fusion) and E15.5 (after fusion) wild-type (WT) and homozygote (Jf/Jf) embryos, immunohistochemically stained with pSmad2 antibody. Scale bar 50 μm. Medial edge epithelium (mee). Graph: comparison of the percentage of epithelial cells positive for nuclear and cytoplasmic localization of pSmad2 in E15.5 wild-type and homozygote palates. c. Coronal sections through the palate of E15.5 wild-type (WT) and homozygote (Jf/Jf) embryos, immunohistochemically stained with antibodies against Ki67 and cleaved caspase-3. Scale bar 50 μm. Graph: comparison of the percentage of epithelial cells positive for cleaved caspase-3 in E15.5 wild-type and homozygote palates. P-values were determined using two-tailed T-test.