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. 2009 Jul 14;10:74. doi: 10.1186/1471-2202-10-74

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Copyright © 2009 Hu et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Western blot analyses of p-ERK, p-CREB and p-ERα protein levels and the effect of Src inhibition on them following cerebral ischemia. SU6656 (SU) and its vehicle (Veh) were administered 20 min before ischemia (I.c.v.) respectively. 24 h reperfusion after 10 min ischemia was performed. (A) Effect of SU on p-ERK (Thr202/Tyr204) in plasma membrane and cytoplasm (M and C) following cerebral ischemia, (B) Effect of SU on p-ERK in nucleus (N) post-ischemia, (C) Role of SU in p-CREB (Ser118) following post-ischemic reperfusion, (D) Role of SU in p-ERα (Ser133) during reperfusion post-ischemia. Bands were scanned and the optical density (O.D.) is presented as fold-increase compared to sham control levels. Data are expressed as mean ± S.D. (n = 4/group), ^#P < 0.05 vs. the respective control group.