Skip to main content
. Author manuscript; available in PMC: 2010 May 15.
Published in final edited form as: J Immunol. 2009 May 15;182(10):6287–6297. doi: 10.4049/jimmunol.0804333

FIGURE 3.

FIGURE 3

Characterization of granzyme protein expression in T cells activated during MLRs. Splenocytes from WT or granzyme B−/− cre-deficient mice were cultured with irradiated BALB/c splenocytes (2000 cGy) in K10 medium supplemented with IL-2 (50 U/ml). MLR cultures were harvested for flow cytometric analysis at various times during activation. T cell expression of granzymes B and C are shown. Results from an individual experiment are shown, gating on CD4+ T cells (A) and CD8+ T cells (B). The percentage of CD4+ T cells positive for granzymes B and C is summarized in C and the percentage of CD8+ T cells positive for granzymes B and C is summarized in D. Summary results (mean ± SD) represent data pooled from three independent experiments.