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. Author manuscript; available in PMC: 2010 May 15.
Published in final edited form as: J Immunol. 2009 May 15;182(10):6287–6297. doi: 10.4049/jimmunol.0804333

FIGURE 4.

FIGURE 4

Quantitative real-time RT-PCR comparing WT and granzyme B−/− cre T cell expression of granzyme C mRNA. CD8+ T cells were cultured in K10 medium with CD3/CD28 beads and RNA was harvested for qRT-PCR at various times during activation. The mean ± SEM expression from three independent experiments is shown as fold change of activated compared with naive T cells (A). The comparative Ct method was used and granzyme C expression was normalized to β-actin. Raw threshold cycles for actin and granzyme C mRNA on day 4 are plotted on an inverted scale in B.