Characterization of constitutive granzyme protein expression in CD8αα+ IELs. IELs were harvested from the intestines of WT and granzyme B−/− cre-deficient mice. Cells were stained and analyzed by flow cytometry for the expression of TCRαβ, TCRγδ, CD8β, and granzyme B. Representative flow plots, gated on CD8α+ IELs, are shown in A. Coexpression of granzymes B and C in WT and granzyme B−/− cre-deficient CD8α+ IELs is shown in B. The percentage of CD8αα− IELs positive for granzymes B and C is summarized in C and D, respectively. Summary results represent data from eight mice per genotype.