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. Author manuscript; available in PMC: 2010 May 15.
Published in final edited form as: J Immunol. 2009 May 15;182(10):6287–6297. doi: 10.4049/jimmunol.0804333

FIGURE 7.

FIGURE 7

NK cell granzyme C protein activation in GzmB−/− cre mice is accelerated. Splenocytes were isolated from WT 129/SvJ or GzmB−/− cre 129/SvJ mice, stained, and analyzed by flow cytometry for the expression of intracellular granzymes B and C at rest (day 0) or after 1, 2, 3, or 4 days of activation with recombinant murine IL-15 (100 ng/ml). Representative flow plots (gated on NKp46+CD3− NK cells) demonstrate the expression of granzyme B (upper panels) and granzyme C (lower panels) in WT (A) or GzmB−/− cre (B) 129/SvJ mice. Notably, in GzmB−/− cre mice, granzyme C protein is activated in a similar fashion as granzyme B in WT mice. The percentage (mean ± SD) of granzyme B- and C-positive NK cells from WT and GzmB−/− cre mice is summarized in C. These results are from three independent experiments.