Table 3.
Partial (5’-end 606-bp) 16S rDNA Sequence Polymorphisms in 10 Nocardia Species
| Strain Identification no. | Identification Based on Comparison with Reference Sequence | % Similarity to Reference Sequence | Site of Nucleotide Polymorphisms (bp Position: Reference Sequence → Isolate Sequence) | 100% Similarity to Another GenBank Sequences Rather Than Reference Sequences (13 Novel Sequence GenBank Accession no.) |
|---|---|---|---|---|
| 04-303-0576 | N. aobensis | 99.67 | 137 G→A | novel sequence1 (FJ172101) |
| 01-320-2714 | N. arthritidis | 99.0 | 132 C→T, 240 G→A, 251 C→T, 341 C→G, 566 A→G, 588 G→T | novel sequence1 (FJ172102) |
| 02-071-3627 | N. asteroides | 99.0 | 133-135 TTC→ACA, 148-150 GAG→TGT | novel sequence1 (FJ172103) |
| 00-194-3516 | N. brasiliensis | 99.84 | 203 T→C | Z36935 |
| 03-273-2825 | N. brasiliensis | 99.67 | 203 T→C, 328 G→A | AY245543 |
| 99-167-2395 | N. brasiliensis | 99.67 | 203 T→C, 328 G→A | AY245543 |
| 00-159-1584 | N. cyriacigeorgica | 99.84 | 576 G→A | novel sequence1 (FJ172112) |
| 04-181-3939 | N. cyriacigeorgica | 99.84 | 576 G→A | |
| 05-111-2308 | N. cyriacigeorgica | 99.84 | 576 G→A | |
| 01-109-2248 | N. farcinica | 99.84 | 67 A→A/G2 | novel sequence1 (FJ172117) |
| 05-053-4454 | N. nova | 99.84 | 86 C→ C/T2 | novel sequence1 (FJ172126) |
| 02-352-3316 | N. nova | 99.84 | 136 G→A/G2 | novel sequence1 (FJ172120) |
| 00-130-2170 | N. nova | 99.84 | 260 A→A/G2 | novel sequence1 (FJ172121) |
| 04-110-3287 | N. nova | 99.67 | 86 C→C/T2, 136 G→A/G2 | novel sequence1 (FJ172125) |
| 00-056-3529 | N. nova | 99.67 | 86 C→T, 136 G→A | AF430030 |
| 01-067-1349 | N. nova | 99.67 | 86 C→T, 136 G→A | |
| 01-097-0996 | N. nova | 99.67 | 86 C→T, 136 G→A | |
| 02-199-2723 | N. nova | 99.67 | 86 C→T, 136 G→A | |
| 00-025-0538 | N. nova | 99.67 | 135 G→T, 148 T→G | AF430032 |
| 00-314-1789 | N. nova | 99.67 | 135 G→T, 148 T→G | |
| 04-150-0614 | N. nova | 99.67 | 135 G→T, 148 T→G | |
| 01-066-1903 | N. nova | 99.51 | 86 C→T, 136 G→A, 578 T→G | novel sequence1 (FJ172122) |
| 02-021-0419 | N. nova | 99.51 | 135 G→T, 148 T→G, 328 A→G | novel sequence1 (FJ172119) |
| 01-114-2816 | N. otitidiscaviarum | 99.85 | 133 A→G | novel sequence1 (FJ172127) |
| ATCC BAQ-278 | N. paucivorans | 99.67 | 33 C-ins3, 37 G-ins3 | AF179865 |
| 03-141-3073 | N. paucivorans | 99.67 | 33 C-ins3, 37 G-ins3 | |
| 03-185-3304 | N. paucivorans | 99.67 | 33 C-ins3, 37 G-ins3 | |
| 03-240-2758 | N. paucivorans | 99.67 | 33 C-ins3, 37 G-ins3 | |
| 05-200-1797 | N. paucivorans | 99.67 | 33 C-ins3, 37 G-ins3 | |
| 97-114-0609 | N. paucivorans | 99.67 | 33 C-ins3, 37 G-ins3 | |
| 97-16298 | N. paucivorans | 99.67 | 33 C-ins3, 37 G-ins3 | |
| 03-310-2776 | N. transvalensis | 99.67 | 402, 403 AG→CA | novel sequence1 (FJ172131) |
1
Sequences of isolates without a match with GenBank sequences are novel sequences.
2
At the specified bp position, both nucleotides were present due to different multiple copies of the 16S rRNA gene.
3
Refers to insertion of the specified base.