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. 2009 Jul 21;17(1):110–122. doi: 10.1016/j.devcel.2009.04.016

Figure 7.

Figure 7

A Population of SNX1-Labeled Endosomes Resides in Close Proximity to the Rab6IP1-labeled TGN

(A) Live-cell imaging shows an incoming carrier containing CD8-CI-MPR labeled with a CD8-FITC antibody colocalizing with a moving, mCherry-Rab6IP1-decorated fiber emanating from the TGN (arrowheads) (Movie S7). Time in seconds. Bar, 12 μm.

(B) Triple labeling of GFP-Rab6IP1, TGN46, and SNX1 shows Rab6IP1 colocalization with the TGN, with numerous SNX1-labeled endosomes being concentrated in close proximity to TGN cisternae. Bar, 28 μm.

(C) Imaging and quantification of the redistribution of vesicles decorated with endogenous SNX1 from the Golgi area towards the cell periphery by suppression of Rab6IP1. Bar, 28 μm. Error bars indicate standard deviation.

(D) Electron micrograph of a Golgi/TGN complex of GFP-Rab6IP1 expressing HeLa cells immunolabeled for GFP (10 nm gold) and endogenous SNX1 (5 nm gold). SNX1 labeling is found in clusters on presumptive TGN tubulo-vesicular profiles (arrowheads). Rab6IP-1 labeling is mainly found on the same TGN profiles (arrows) and occasionally colocalizes with SNX1. Scale bar represents 100 nm.