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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Arthritis Rheum. 2008 Aug;58(8):2296–2306. doi: 10.1002/art.23610

Figure 3.

Figure 3

Levels of matrix metalloproteinase (MMP) mRNA, collagenase activity, and MMP-3 activity in fibroblast-like synoviocytes (FLS) from DA rats and DA.F344(Cia5d) rats. A, Messenger RNA levels of MMP-1, MMP-2, MMP-3, MMP-9, MMP-13, membrane type 1 (MT1)–MMP, and MT2-MMP were not significantly different in FLS cell lines from DA rats and FLS cell lines from DA.F344(Cia5d) rats, as determined by quantitative polymerase chain reaction. B, Collagenase activity was nearly identical in the supernatants of FLS cultures from DA rats (n=7) and DA.F344(Cia5d) rats (n = 7). Values in A and B are the mean and SD. C, Casein zymography revealed similar levels of MMP-3 in the supernatants of FLS cultures from DA rats (n = 4) and DA.F344(Cia5d) rats (n = 4). ΔCt = change in threshold cycle.