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. 2009 May 22;18(16):2963–2974. doi: 10.1093/hmg/ddp235

Figure 5.

Figure 5.

Confocal imaging of HeLa cells expressing wild-type and mutant Uromodulin. Orthogonal reconstructions of Z-stacks of transfected cells fixed and stained for Uromodulin (green) and Calnexin (red). Co-localization of Uromodulin and Calnexin yields a yellow signal. Nuclei are stained with DAPI (blue). Scale bars: 10 µm. (A) Wild-type, 12 h post-transfection; (B) C32W Uromodulin, 24 h post-transfection; (C) C126R Uromodulin, 24 h post-transfection; (D) C223R Uromodulin, 24 h post-transfection; (E) G488R Uromodulin, 12 h post-transfection. N128S Uromodulin showed an identical pattern to that of C126R Uromodulin, and D196N Uromodulin showed a similar pattern to that of C223R Uromodulin (data not shown).