Figure 2.

Endogenous ISD11 forms a complex with ISCS in vivo. Co-immunoprecipitation of extracts prepared from HeLa cell lysate was performed as described in Materials and Methods. Cell lysates were first immunoprecipitated with anti-ISCS antibody (A; Lane 2) or anti-ISD11 antibody (B; Lane 2), or IgG as negative control (A and B; Lane 1) followed by western blot analysis using anti-ISD11 (A) or anti-ISCS antibody (B), respectively.