Skip to main content
. Author manuscript; available in PMC: 2010 Apr 21.
Published in final edited form as: Biochemistry. 2009 Apr 21;48(15):3378–3386. doi: 10.1021/bi900057g

Figure 8. Effect of pH on Rescue of Abl R367A by Imidazole and 2-Aminoimidazole.

Figure 8

A) Activation by imidazole (pKa = 7.0) and B) 2-aminoimidazole (pKa = 8.5) of R367A AblKD at pH 6.8 and 8.2 were fit to equation 1. Reactions were performed at 30 ° in 100 mM HEPES, 10 mM MgCl2, 1 mM ATP, 440 µM Abltide, 0.1 U/µL pyruvate kinase, 0.05 U/µL lactate dehydrogenase, 1 mM phosphoenolpyruvate, 150 µM NADH, and 0.5 mM Na3VO4 at the indicated pH.