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Validation of mRAP data by Northern blot analysis. Fractions containing small RNAs (0.5–0.8 μg per lane) isolated from (left lanes) male or (right lanes) female mouse gonads at E13.5 were subjected to Northern analysis with LNA probes specific for the indicated miRNAs or U6 RNA. The signal intensity for each miRNA normalized by that of U6 RNA is shown at the bottom of each lane. (Closed and open arrowheads) The positions corresponding to 24 and 19 nt, respectively.
