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. 2009 Aug;15(8):1507–1514. doi: 10.1261/rna.1418309

FIGURE 4.

FIGURE 4.

Expression of miR-29b and miR-143 in PGCs of mouse embryos at E13.5. (A) Male (M) and female (F) embryonic gonads were recovered at E13.5 from dams injected with DMSO or busulfan and were subjected to whole-mount in situ hybridization with a probe specific for Oct4 mRNA. (B) Fractions containing small RNAs isolated from female or male gonads treated as in A were subjected to Northern blot analysis with probes specific for miR-29b or miR-143, respectively. The same blots were also subjected to hybridization with a probe specific for U6 RNA. (Closed and open arrowheads) The positions corresponding to 24 and 19 nt, respectively. Embryos exposed to (D) DMSO or (B) busulfan.