Table 1.
Proportions of virions expressing each fluorescent marker
| Cells transfected with | No. CeFP particles analyzed | % CeFP+ + YFP+ | % CeFP+ + mCherry+ | % CeFP+ + YFP+ + mCherry+ | RNA labeling efficiency* |
|---|---|---|---|---|---|
| 1. Bgl-mCherry + GagCeFP-BglSL | |||||
| Exp. a | 6092 | 0.00 | 93.4 | 0.05 | 93.5 |
| Exp. b | 2861 | 0.00 | 93.8 | 0.07 | 93.9 |
| 2. Bgl-mCherry + GagCeFP-MSSL | |||||
| Exp. a | 2136 | 0.37 | 0.00 | 0.00 | 0.37 |
| Exp. b | 2042 | 0.05 | 0.10 | 0.00 | 0.15 |
| 3. MS2-YFP + GagCeFP-MSSL | 1902 | 94.9 | 0.00 | 0.00 | 94.9 |
| 4. MS2-YFP + GagCeFP-BglSL | |||||
| Exp. a | 3872 | 0.46 | 0.00 | 0.00 | 0.46 |
| Exp. b | 2826 | 0.21 | 0.00 | 0.00 | 0.21 |
| 5. MS2-YFP + Bgl-mCherry + GagCeFP-MSSL + GagCeFP-BglSL | |||||
| Exp. a | 1753 | 19.7 | 30.2 | 44.0 | 94.0 |
| Exp. b | 3220 | 24.6 | 22.8 | 46.3 | 93.7 |
| 6. MS2-YFP + Bgl-mCherry + GagCeFP-MSSL + CDIS-GagCeFP-BglSL | |||||
| Exp. a | 1341 | 39.1 | 34.4 | 21.0 | 94.5 |
| Exp. b | 2003 | 46.8 | 27.8 | 19.3 | 93.9 |
| 7. MS2-YFP + Bgl-mCherry + 6GDIS-GagCeFP-MSSL + 6CDIS-GagCeFP-BglSL | |||||
| Exp. a | 498 | 5.2 | 17.1 | 69.5 | 91.8 |
| Exp. b | 1294 | 6.3 | 15.2 | 69.6 | 91.1 |
*RNA labeling efficiency is the sum of the 3 previous columns, i.e., %CeFP+ + YFP+, %CeFP+ + mCherry+, and %CeFP+ +YFP+ + mCherry+.