Table 2.

A. marginale infection levels in D. variabilis male guts and salivary glands after RNAi of selected tick genes.

Experimental group	Infection levels in guts after AF (A. marginale/tick ± SE)	Infection levels in guts after TF (A. marginale /tick ± SE)	Infection levels in salivary glands after TF (A. marginale/tick ± SE)
GST	5 ± 15*	99,060 ± 68462	2 ± 0*
vATPase	81 ± 5*	795 ± 227	247 ± 205
SelM	389,095 ± 282048	1,451 ± 443	2 ± 0*
Subolesin	814 ± 122	1,517 ± 1025	2 ± 0*
Saline control	40,579 ± 6993	28,252 ± 27788	287 ± 144

The A. marginale infection levels were analyzed in D. variabilis male ticks after RNAi. Salivary glands and/or guts were dissected from 5 ticks after AF and TF and DNA was used for quantitative msp4 PCR to determine A. marginale infection levels. Infection levels in tick guts and salivary glands were expressed as average ± SE and compared between dsRNA and saline injected ticks by Student's t-test (*P ≤ 0.05).