Table 2.
Metabolic characteristics of nerve-repair grafted and control medial gastrocnemius muscle with or without synergistic muscles intact in 6-mo-old male Fischer 344 rats
| Experimental Group | P Value | ||||||
|---|---|---|---|---|---|---|---|
| Sham control |
Nerve-repair grafted |
Synergist-ablated control |
Synergist-ablated nerve-repair grafted |
Effect of grafting |
Effect of ablation |
Effect of grafting × ablation |
|
| Body weight, g | 371 ± 9 (10) |
380 ± 5 (10) |
346 ± 5c (10) |
356 ± 5d (10) |
NS | 0.003 | NS |
| Muscle protein content, mg protein/g tissue |
232 ± 12 (8) |
205 ± 11 (7) |
153 ± 10c (9) |
173 ± 7d,e (10) |
NS | < 0.001 | 0.024 |
| Glycogen content, µmol/g muscle |
36 ± 2 (9) |
30 ± 1a (7) |
12 ± 1c (10) |
14 ± 1b,d,e (10) |
NS | < 0.0001 | 0.0002 |
| Citrate synthase, µmol · µg−1 · min−1 |
176 ± 18 (6) |
139 ± 3a (5) |
175 ± 4 (9) |
149 ± 8b,e (10) |
0.002 | NS | NS |
| Hexokinase, U/mg protein | 25 ± 6 (8) |
58 ± 5a (7) |
47 ± 2c (10) |
48 ± 1d,e (10) |
< 0.0001 | NS | < 0.0001 |
| GLUT-4 protein, %sham control |
100 ± 4 (10) |
101 ± 5 (10) |
170 ± 15c (8) |
175 ± 12d,e (8) |
NS | < 0.0001 | NS |
| GLUT-1 protein, %sham control |
100 ± 9 (8) |
143 ± 19 (9) |
85 ± 11 (5) |
137 ± 24 (5) |
0.012 | NS | NS |
Values are means ± SE; nos. in parentheses is no. of rats for each group.
a
Nerve-repair grafted is significantly different from sham control group, P ≤ 0.05.
b
Synergist-ablated nerve-repair grafted is significantly different from synergist-ablated control group, P ≤ 0.05.
c
Synergist-ablated control is significantly different from sham control group, P < 0.05.
d
Synergist-ablated nerve-repair grafted is significantly different from nerve-repair grafted group, P ≤ 0.05.
e
Synergist-ablated nerve-repair grafted is significantly different from sham control group, P ≤ 0.05.