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. Author manuscript; available in PMC: 2010 Jun 15.
Published in final edited form as: J Immunol. 2009 Jun 15;182(12):7451–7458. doi: 10.4049/jimmunol.0804296

FIGURE 3.

FIGURE 3

HSP90 inhibition by GA or RA Did Not Alter MHC Class II Expression. PriessGAD cells were treated with DMSO as a control, RA, or GA for 18 hours. (A) Following drug treatment, cells were lysed in 1% Triton-X 100 buffer. Cell lysates were analyzed by Western immunoblotting for expression of MHC class II DRα chain (left) or MHC class II DRαβ dimers (right) compared to actin control. In all panels, the intervening lanes were removed between samples. Results shown are representative of 3 separate experiments. The table depicts the average relative expression of MHC class II DRα and DRαβ dimers as measured by densitometry using data from 3 separate experiments. (B) Drug treated cells were also fixed in 1% paraformaldehyde and stained for surface MHC class II expression (left). For total MHC class II expression (right), cells were fixed, permeabilized, then stained for total MHC class II expression. MHC class II expression was detected by flow analysis. Thick black line indicates control cells, thin gray line indicates GA treated cells, and the dashed black line indicates RA treated cells. Results shown are representative of 3 separate experiments.