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. 2009 Jun;21(6):1747–1761. doi: 10.1105/tpc.108.062968

Figure 7.

Figure 7.

Effect of a bZIP53 T-DNA Insertion Mutant on Seed-Specific Gene Regulation.

(A) mRNA levels detected of the seed maturation genes indicated by qRT-PCR in Col wild-type and bzip53 siliques at different stages of development. Lane 1, 0 d after flowering (DAF); lane 2, 2 DAF; lane 3, 4 DAF; lane 4, 6 DAF; lane 5, 9 DAF; lane 6, 12 DAF; lane 7, 15 DAF; lane 8, ≥18 DAF. Data are normalized using UBIQUITIN expression values. Average values and standard errors from at least two technical replicates are shown.

(B) Transient expression by microparticle bombardment of Arabidopsis leaves from the wild type (white bars) and plants with increased (Pro35S:bZIP53; gray bars) or decreased (bzip53; black bars) expression of bZIP53. Effector constructs containing bZIP10 or ABI3 under the control of a 35S promoter (Pro35S) and a GUS reporter gene under the control of the 2S2 promoter were used. The x axis values are expressed as GUS activity relative to NAN (Kirby and Kavanagh, 2002). Average values and standard errors from four replicates and two different experiments are shown.