Figure 2.

WDR5a Represses the Floral Transition in Arabidopsis.

(A) WDR5a gene structure. Exons are represented by filled boxes; the start and stop codons are marked as ATG and TAA, respectively. The 232-bp region used to knock down WDR5a expression is indicated by broken lines.

(B) wdr5a (RNAi) lines grown in long days. Col is the parental accession used in the RNAi-mediated WDR5a suppression.

(C) Flowering times of wdr5a (RNAi) lines grown in long days. The total number of primary rosette and cauline leaves at flowering was counted, and for each line, 16 plants were scored. The values shown are means ± sd.

(D) Relative mRNA levels of WDR5a and WDR5b in seedlings of wdr5a (RNAi) lines quantified by real-time PCR. Relative expression to parental Col is presented, with sd for three quantitative PCR replicates ([D] and [E]).

(E) Relative FLC mRNA levels in seedlings of wdr5a (RNAi) lines quantified by real-time PCR.

[See online article for color version of this figure.]