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. 2009 Jun;21(6):1813–1829. doi: 10.1105/tpc.109.066399

Figure 5.

Figure 5.

Analyses of the bat5 Mutant, Cultured Pro35S:amiBAT5 Arabidopsis Cells, and Stably Transformed Pro35S:amiBAT5 Plants.

(A) to (C) Steady state level of BAT5 mRNA in three different plants of the bat5 mutant (A), cultured Pro35S:amiBAT5 Arabidopsis cells (B), and stably transformed Pro35S:amiBAT5 plants (C). Relative gene expression values are given in comparison with the wild-type (Col-0) plants or wild-type cells (wild type = 1). Means ± sd (n = 3). nd, not detectable.

(D) Contents of GSs (nmol/mg dry weight) in bat5 mutant plants in comparison with the wild type (Col-0). Means ± sd (n = 5).

(E) Contents of GSs (nmol/mg dry weight) in Pro35S:amiBAT5 cultured Arabidopsis cell lines in comparison with wild-type cells. The levels of long-chained aliphatic GSs were below the detection limit in both wild-type cells extracts and in Pro35S:amiBAT5 cells. Means ± sd (n = 3).

(F) Contents of GSs (nmol/mg dry weight) in Pro35S:amiBAT5 stably transformed Arabidopsis plants in comparison with cultured wild-type cells. Means ± sd (n = 5). Asterisk indicates significant difference in comparison to the wild-type Col-0 (Student's t test, P < 0.05).