Table 1. Identification and characterization of selected mutants.
ORF/STM mutant | Function | Gene name | Slow killing | Cell Adherence | Swimming | Swarming |
PA0173/TB0173s | probable methylesterase for chemotaxis | cheB2 | − | − | − | − |
PA0260/TB0260s | hypothetical protein | − | + | − | + | |
PA0946/TB0946s | hypothetical protein | − | + | + | + | |
PA2478/TB2478s | probable thiol∶disulfide interchange protein | dsbD2 | + | + | + | + |
PA2585/TB2585s | endonuclease UvrABC subunit C | uvrC | − | + | + | + |
PA2588/TB2588s | probable transcriptional regulator | + | − | + | − | |
PA2769/TB2769s | hypothetical protein | + | − | + | + | |
PA3080/TB3080s | hypothetical protein | − | − | + | − | |
PA4380/TB4380s | probable histidine kinase sensor | colS | − | − | + | + |
PA4554/TB4554s | type 4 fimbrial biogenesis protein | pilY1 | − | +/− | + | + |
PA4953/TB4953s | flagellar motor protein | motB | + | − | − | − |
PA5479/TB5479s | proton-glutamate symporter | gltP | + | − | − | + |
ORF PA number, function and gene name annotations were obtained using the Pseudomonas genome database version 2 (http://v2.pseudomonas.com/index.jsp).
+ indicates that the strain exhibited a phenotype not significantly different from the wild type TB isolate, whereas − indicates a reduced phenotype. +/− indicates a reduction at the limit of significance.