Table 3. Oligonucleotides used in this study.

Oligonucleotide name	Sequence#	Position*
IR1f	5′-GCTGCGTTCGGTCAAGGTTC-3′	130 bp downstream from the IR upstream Gmr in pTnMod-OGm
IR1r	5′-CATTTTTTGTGATGCTCGTCAGGG-3′	100 bp upstream of the IR upstream oriR in pTnMod-OGm
CheIN1	5′-GACCTGATCAAGCAGCACGC-3′	120 bp downstream from the cheB2 ATG
CheIN2	5′-CGAACATCACGTCCACTGCC-3′	260 bp upstream from the cheB2 stop codon
ChebUP	5′-AGCTGAAGAACGACACCGTGC-3′	100 bp upstream of the cheB2 ATG
ChebDW	5′-TGCGGGGGGGCGAAAAAGC-3′	24 bp downstream the cheB2 stop codon
Che1INU	5′-GCTGGCCCTGAGACCCGA-3′	131 bp downstream from the cheB1 ATG
Che1INR	5′-GATGTCGCCGTCCTCTGCT-3′	363 bp upstream from the cheB1 stop codon
Che1UP	5′-AAGCCACTCGGCAAGATGTTG-3′	154 bp upstream of the cheB1 ATG
Che1DW	5′-GCGACGAACGCCAGGATG-3′	115 bp downstream the cheB1 stop codon
B2CTX1	5′-CGTAAGCTTGCAGTACGCCTATGCCTACC-3′	393 bp upstream of the mcpA (PA0180) ATG
B2CTX2	5′-CGTGAATTCCAGCTTCAGCAGCAGGGGC-3′	35 bp downstream of the mcpA ATG
B2CTX3	5′-TAACGAATTCTCCTGGTGAAGTCCCTGGTGG-3′	130 bp upstream of the cheB2 ATG
B2CTX4	5′-TAACAAGCTTACACAATGATGGCGAGCAGGC-3′	100 bp downstream of the cheB2 stop codon

*: Gm^r: Gentamycin resistance cassette.

^#restriction sites within oligonucleotides are with italics.